BAIT
P2RX1
P2X1
purinergic receptor P2X, ligand-gated ion channel, 1
GO Process (8)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ATP2C1
ATP2C1A, BCPM, HHD, PMR1, SPCA1, hSPCA1, HUSSY-28
ATPase, Ca++ transporting, type 2C, member 1
GO Process (15)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- Golgi calcium ion homeostasis [IMP]
- Golgi calcium ion transport [IMP]
- actin cytoskeleton reorganization [IMP]
- calcium ion transmembrane transport [IDA, IMP]
- calcium ion transport [IDA, IMP]
- calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules [IMP]
- cellular calcium ion homeostasis [IDA, IMP]
- cellular manganese ion homeostasis [IDA]
- epidermis development [IMP]
- ion transmembrane transport [TAS]
- manganese ion transmembrane transport [IDA]
- manganese ion transport [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- signal transduction [IMP]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.955463107 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.955463107, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID