BAIT
GCNT2
CCAT, CTRCT13, GCNT2C, GCNT5, IGNT, II, NACGT1, NAGCT1, ULG3, bA360O19.2, bA421M1.1, RP11-421M1.1
glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group)
GO Process (13)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- glycosaminoglycan biosynthetic process [TAS]
- maintenance of lens transparency [IMP]
- multicellular organismal development [TAS]
- negative regulation of cell-substrate adhesion [IMP, ISS]
- positive regulation of ERK1 and ERK2 cascade [IMP, ISS]
- positive regulation of cell migration [IMP, ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of epithelial to mesenchymal transition [IMP, ISS]
- positive regulation of heterotypic cell-cell adhesion [IMP, ISS]
- positive regulation of protein kinase B signaling [IMP, ISS]
- posttranscriptional regulation of gene expression [IMP, ISS]
- protein glycosylation [IMP]
- transforming growth factor beta receptor signaling pathway [IMP, ISS]
Gene Ontology Molecular Function
Homo sapiens
PREY
PGAM2
GSD10, PGAM-M, PGAMM
phosphoglycerate mutase 2 (muscle)
GO Process (6)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.992314205 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.992314205, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID