BAIT
P2RY1
P2Y1
purinergic receptor P2Y, G-protein coupled, 1
GO Process (9)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISS]
- blood coagulation [TAS]
- cell surface receptor signaling pathway [TAS]
- platelet activation [TAS]
- positive regulation of ERK1 and ERK2 cascade [ISS]
- positive regulation of cytosolic calcium ion concentration [ISS]
- positive regulation of protein phosphorylation [ISS]
- protein localization to plasma membrane [ISS]
- signal transduction involved in regulation of gene expression [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DHCR24
DCE, Nbla03646, SELADIN1, seladin-1
24-dehydrocholesterol reductase
GO Process (11)
GO Function (4)
GO Component (4)
Gene Ontology Biological Process
- apoptotic process [NAS]
- cell cycle arrest [NAS]
- cholesterol biosynthetic process [IMP, ISS, TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- oxidation-reduction process [IMP]
- regulation of neuron death [NAS]
- response to oxidative stress [IEP]
- skin development [ISS]
- small molecule metabolic process [TAS]
- tissue development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.967057712 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.967057712, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID