BAIT

INSR

CD220, HHF5
insulin receptor
GO Process (28)
GO Function (13)
GO Component (9)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interaction between the p21ras GTPase activating protein and the insulin receptor.

Pronk GJ, Medema RH, Burgering BM, Clark R, McCormick F, Bos JL

We investigated the involvement of the p21ras-GTPase activating protein (GAP) in insulin-induced signal transduction. In cells overexpressing the insulin receptor, we did not observe association between GAP and the insulin receptor after insulin treatment nor the phosphorylation of GAP on tyrosine residues. However, after insulin treatment in the presence of the phosphotyrosine phosphatase inhibitor phenylarsine oxide (PAO), 5-10% of GAP ... [more]

J. Biol. Chem. Nov. 25, 1992; 267(33);24058-63 [Pubmed: 1331107]

Throughput

  • Low Throughput

Curated By

  • BioGRID