BAIT
PIGT
MCAHS3, NDAP, PNH2, CGI-06
phosphatidylinositol glycan anchor biosynthesis, class T
GO Process (4)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
JUP
ARVD12, CTNNG, DP3, DPIII, PDGB, PKGB
junction plakoglobin
GO Process (20)
GO Function (9)
GO Component (17)
Gene Ontology Biological Process
- adherens junction organization [TAS]
- bundle of His cell to Purkinje myocyte communication [IMP]
- cell junction assembly [TAS]
- cell migration [IMP]
- cell-cell junction organization [TAS]
- cellular response to indole-3-methanol [IDA]
- cytoskeletal anchoring at plasma membrane [NAS]
- desmosome assembly [IDA, IMP]
- detection of mechanical stimulus [IDA]
- endothelial cell-cell adhesion [ISS]
- establishment of protein localization to plasma membrane [IMP]
- positive regulation of canonical Wnt signaling pathway [IC]
- positive regulation of protein import into nucleus [IDA]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IBA]
- regulation of cell fate specification [IBA]
- regulation of cell proliferation [IDA]
- regulation of heart rate by cardiac conduction [IMP]
- single organismal cell-cell adhesion [IDA, IMP]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- catenin complex [IDA]
- cell-cell adherens junction [IDA]
- cell-cell junction [IDA]
- cytoplasm [IMP]
- cytoplasmic side of plasma membrane [ISS]
- cytoskeleton [ISS]
- cytosol [ISS]
- desmosome [IDA]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- gamma-catenin-TCF7L2 complex [IDA]
- hemidesmosome [ISS]
- intercalated disc [IDA]
- nucleus [IMP]
- plasma membrane [IDA, TAS]
- protein-DNA complex [IDA]
- zonula adherens [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.867219848 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.867219848, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID