An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The regulators of G protein signaling (RGS) domains of RGS4, RGS10, and GAIP retain GTPase activating protein activity in vitro.

Popov S, Yu K, Kozasa T, Wilkie TM

Regulators of G protein signaling (RGS) proteins accelerate GTP hydrolysis by Gi but not by Gs class alpha-subunits. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. We have demonstrated that the RGS domains of RGS4, RGS10, and GAIP retain GTPase accelerating activity with the Gi class substrates Gialpha1, Goalpha, and Gzalpha in vitro. No regulatory ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jul. 08, 1997; 94(14);7216-20 [Pubmed: 9207071]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GNAI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein beta/gamma-subunit complex binding [IBA]G-protein coupled serotonin receptor binding [IBA]GTP binding [ISS]GTPase activity [IBA, TAS]protein binding [IPI]signal transducer activity [IBA]

Gene Ontology Cellular Component

RGS10