BAIT
AHR
bHLHe76
aryl hydrocarbon receptor
GO Process (15)
GO Function (11)
GO Component (6)
Gene Ontology Biological Process
- apoptotic process [TAS]
- blood vessel development [NAS]
- circadian regulation of gene expression [ISS]
- intracellular receptor signaling pathway [IDA]
- negative regulation of transcription, DNA-templated [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IBA]
- positive regulation of transcription, DNA-templated [ISS]
- regulation of B cell proliferation [IDA]
- regulation of gene expression [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA]
- response to toxic substance [IDA]
- response to xenobiotic stimulus [IDA]
- transcription from RNA polymerase II promoter [IDA]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function- DNA binding [IDA, TAS]
- E-box binding [ISS]
- Hsp90 protein binding [IDA]
- enhancer binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein dimerization activity [TAS]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA, TAS]
- E-box binding [ISS]
- Hsp90 protein binding [IDA]
- enhancer binding [IDA]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- protein binding [IPI]
- protein dimerization activity [TAS]
- protein heterodimerization activity [TAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
SH2D4A
PPP1R38, SH2A
SH2 domain containing 4A
GO Process (1)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.965978848 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.965978848, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID