CDC42
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- GTP catabolic process [TAS]
- Golgi organization [ISS]
- T cell costimulation [TAS]
- actin cytoskeleton organization [IDA]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of Golgi localization [ISS]
- establishment or maintenance of cell polarity [TAS]
- innate immune response [TAS]
- macrophage differentiation [TAS]
- muscle cell differentiation [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of protein complex assembly [IPI]
- organelle transport along microtubule [ISS]
- positive regulation of cytokinesis [IMP]
- positive regulation of muscle cell differentiation [TAS]
- positive regulation of pseudopodium assembly [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of filopodium assembly [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [ISS]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- midbody [IDA]
- mitotic spindle [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- plasma membrane [IDA, TAS]
- spindle midzone [IDA]
PLD1
Gene Ontology Biological Process
- Ras protein signal transduction [TAS]
- chemotaxis [TAS]
- glycerophospholipid biosynthetic process [TAS]
- phosphatidic acid biosynthetic process [TAS]
- phosphatidylglycerol biosynthetic process [TAS]
- phospholipid metabolic process [TAS]
- small GTPase mediated signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Activation of phospholipase D1 by Cdc42 requires the Rho insert region.
Members of the Rho subfamily of GTP-binding proteins are implicated in the regulation of phospholipase D (PLD). In the present study, we demonstrate a physical association between a Rho family member, Cdc42, and PLD1. Binding of Cdc42 to PLD1 and subsequent activation are GTP-dependent. Although binding of Cdc42 to PLD1 does not require geranylgeranylation, activation of PLD1 is dependent on ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CDC42 PLD1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 3332091 | |
| CDC42 PLD1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID