BAIT
UBXN6
UBXD1, UBXDC2
UBX domain protein 6
GO Process (0)
GO Function (1)
GO Component (3)
Gene Ontology Molecular Function
Homo sapiens
PREY
MAP3K5
ASK1, MAPKKK5, MEKK5, RP3-325F22.4
mitogen-activated protein kinase kinase kinase 5
GO Process (12)
GO Function (8)
GO Component (2)
Gene Ontology Biological Process
- JNK cascade [IDA]
- MAPK cascade [IDA]
- activation of JUN kinase activity [TAS]
- activation of MAPKK activity [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- apoptotic signaling pathway [TAS]
- cellular response to hydrogen peroxide [IDA]
- intrinsic apoptotic signaling pathway in response to oxidative stress [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of neuron death [IGI]
- protein phosphorylation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.967631925 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.967631925, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID