An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Binding of mouse and human fibulin-2 to extracellular matrix ligands.

Sasaki T, Goehring W, Pan TC, Chu ML, Timpl R

Recombinant mouse and human fibulin-2 were obtained as disulfide-bonded trimers from transfected kidney cell clones and used in solid phase, biosensor and radioligand binding assays. Strong binding occurred with fibronectin and required calcium. A distinct interaction was also observed with nidogen but this was only partially blocked by EDTA. Distinctly weaker affinities were detected for collagen IV, perlecan and the ... [more]

J. Mol. Biol. Dec. 15, 1995; 254(5);892-9 [Pubmed: 7500359]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

FN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

collagen binding [NAS]heparin binding [NAS]integrin binding [IDA, IMP]protease binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

FBLN2