PREY

LEF1

LEF-1, TCF10, TCF1ALPHA, TCF7L3
lymphoid enhancer-binding factor 1
GO Process (61)
GO Function (19)
GO Component (5)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Microphthalmia-associated transcription factor interacts with LEF-1, a mediator of Wnt signaling.

Yasumoto K, Takeda K, Saito H, Watanabe K, Takahashi K, Shibahara S

Wnt signals regulate differentiation of neural crest cells through the beta-catenin associated with a nuclear mediator of the lymphoid-enhancing factor 1 (LEF-1)/T-cell factors (TCFs) family. Here we show the interaction between the basic helix-loop-helix and leucine-zipper region of microphthalmia-associated transcription factor (MITF) and LEF-1. MITF is essential for melanocyte differentiation and its heterozygous mutations cause auditory-pigmentary syndromes. Functional cooperation of ... [more]

EMBO J. Jun. 03, 2002; 21(11);2703-14 [Pubmed: 12032083]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MITF LEF1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
MITF LEF1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
MITF LEF1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID