PIAS4
Gene Ontology Biological Process
Gene Ontology Molecular Function
LEF1
Gene Ontology Biological Process
- B cell proliferation [IBA]
- BMP signaling pathway [IBA]
- T-helper 1 cell differentiation [ISS]
- Wnt signaling pathway [IDA]
- alpha-beta T cell differentiation [IBA]
- apoptotic process involved in morphogenesis [IBA]
- canonical Wnt signaling pathway [IDA, IMP]
- cell chemotaxis [IDA]
- cellular response to cytokine stimulus [IMP]
- cellular response to interleukin-4 [IDA]
- chorio-allantoic fusion [IBA]
- dentate gyrus development [IBA]
- embryonic limb morphogenesis [IBA]
- epithelial to mesenchymal transition [ISS]
- eye pigmentation [IBA]
- face morphogenesis [IBA]
- forebrain neuroblast division [IBA]
- forebrain neuron differentiation [IBA]
- forebrain radial glial cell differentiation [IBA]
- formation of radial glial scaffolds [IBA]
- histone H3 acetylation [IMP]
- histone H4 acetylation [IMP]
- hypothalamus development [IBA]
- mammary gland development [IBA]
- muscle fiber development [IBA]
- negative regulation of DNA binding [IDA]
- negative regulation of apoptotic process [IMP]
- negative regulation of apoptotic process in bone marrow [IMP]
- negative regulation of canonical Wnt signaling pathway [IMP]
- negative regulation of cell-cell adhesion [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of estrogen receptor binding [IDA]
- negative regulation of interleukin-13 production [IDA]
- negative regulation of interleukin-4 production [IDA]
- negative regulation of interleukin-5 production [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- neural crest cell migration [IBA]
- neutrophil differentiation [IMP]
- odontoblast differentiation [IBA]
- osteoblast differentiation [IEP]
- palate development [ISS]
- paraxial mesoderm formation [IBA]
- patterning of blood vessels [IBA]
- positive regulation by host of viral transcription [IDA]
- positive regulation of cell cycle process [IDA]
- positive regulation of cell growth [IMP]
- positive regulation of cell migration [IDA, IMP]
- positive regulation of cell proliferation [IDA]
- positive regulation of cell proliferation in bone marrow [IMP]
- positive regulation of cell-cell adhesion [IDA]
- positive regulation of epithelial to mesenchymal transition [IMP]
- positive regulation of gene expression [IDA]
- positive regulation of granulocyte differentiation [IDA, IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- regulation of striated muscle tissue development [IBA]
- somitogenesis [IBA]
- sprouting angiogenesis [IBA]
- steroid hormone mediated signaling pathway [IDA]
- trachea gland development [IBA]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- C2H2 zinc finger domain binding [IPI]
- DNA binding [ISS]
- DNA binding, bending [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- armadillo repeat domain binding [IPI]
- beta-catenin binding [IDA, IPI, TAS]
- chromatin binding [IBA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IMP]
- enhancer binding [IDA]
- estrogen receptor activity [IDA]
- estrogen receptor binding [IDA]
- gamma-catenin binding [IPI]
- histone binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- C2H2 zinc finger domain binding [IPI]
- DNA binding [ISS]
- DNA binding, bending [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IBA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- armadillo repeat domain binding [IPI]
- beta-catenin binding [IDA, IPI, TAS]
- chromatin binding [IBA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IMP]
- enhancer binding [IDA]
- estrogen receptor activity [IDA]
- estrogen receptor binding [IDA]
- gamma-catenin binding [IPI]
- histone binding [IPI]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies.
The Wnt-responsive transcription factor LEF1 can activate transcription in association with beta-catenin and repress transcription in association with Groucho. In search of additional regulatory mechanisms of LEF1 function, we identified the protein inhibitor of activated STAT, PIASy, as a novel interaction partner of LEF1. Coexpression of PIASy with LEF1 results in potent repression of LEF1 activity and in covalent modification ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
PIAS4 LEF1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
PIAS4 LEF1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID