BAIT

KCNE1

ISK, JLNS, JLNS2, LQT2/5, LQT5, MinK
potassium voltage-gated channel, Isk-related family, member 1
Homo sapiens
PREY

KCNH2

ERG-1, ERG1, H-ERG, HERG, HERG1, Kv11.1, LQT2, SQT1
potassium voltage-gated channel, subfamily H (eag-related), member 2
GO Process (19)
GO Function (9)
GO Component (3)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A minK-HERG complex regulates the cardiac potassium current I(Kr).

McDonald TV, Yu Z, Ming Z, Palma E, Meyers MB, Wang KW, Goldstein SA, Fishman GI

MinK is a widely expressed protein of relative molecular mass approximately 15K that forms potassium channels by aggregation with other membrane proteins. MinK governs ion channel activation, regulation by second messengers, and the function and structure of the ion conduction pathway. Association of minK with a channel protein known as KvLQT1 produces a voltage-gated outward K+ current (I[sK]) resembling the ... [more]

Nature Jul. 17, 1997; 388(6639);289-92 [Pubmed: 9230439]

Throughput

  • Low Throughput

Curated By

  • BioGRID