BAIT
RUNX3
AML2, CBFA3, PEBP2aC, RP3-398I9.1
runt-related transcription factor 3
GO Process (7)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- negative regulation of cell cycle [ISS]
- negative regulation of epithelial cell proliferation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- peripheral nervous system neuron development [TAS]
- protein phosphorylation [IDA]
- regulation of transcription, DNA-templated [NAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TLE1
ESG, ESG1, GRG1, RP11-203D19.1
transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
GO Process (9)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
- Notch signaling pathway [TAS]
- multicellular organismal development [TAS]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- negative regulation of Wnt signaling pathway [NAS]
- negative regulation of anoikis [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- organ morphogenesis [TAS]
- positive regulation of gene expression [IMP]
- signal transduction [TAS]
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors.
The mammalian AML/CBFalpha runt domain (RD) transcription factors regulate hematopoiesis and osteoblast differentiation. Like their Drosophila counterparts, most mammalian RD proteins terminate in a common pentapeptide, VWRPY, which serves to recruit the corepressor Groucho (Gro). Using a yeast two-hybrid assay, in vitro association and pull-down experiments, we demonstrate that Gro and its mammalian homolog TLE1 specifically interact with AML1 and ... [more]
Proc. Natl. Acad. Sci. U.S.A. Sep. 29, 1998; 95(20);11590-5 [Pubmed: 9751710]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TLE1 RUNX3 | Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe. | Low | - | BioGRID | - |
Curated By
- BioGRID