PREY

CD4

CD4mut
CD4 molecule
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Restricted receptor segregation into membrane microdomains occurs on human T cells during apoptosis induced by galectin-1.

Pace KE, Lee C, Stewart PL, Baum LG

Galectin-1 induces apoptosis of human thymocytes and activated T cells by an unknown mechanism. Apoptosis is a novel function for a mammalian lectin; moreover, given the ubiquitous distribution of the oligosaccharide ligand recognized by galectin-1, it is not clear how susceptibility to and signaling by galectin-1 is regulated. We have determined that galectin-1 binds to a restricted set of T ... [more]

J. Immunol. Oct. 01, 1999; 163(7);3801-11 [Pubmed: 10490978]

Throughput

  • Low Throughput

Curated By

  • BioGRID