CLOCK
Gene Ontology Biological Process
- DNA damage checkpoint [ISO]
- cellular response to ionizing radiation [ISO]
- circadian regulation of gene expression [IMP, ISO]
- circadian rhythm [IDA, IMP]
- histone acetylation [IMP, ISO]
- negative regulation of glucocorticoid receptor signaling pathway [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of inflammatory response [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISO]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- regulation of hair cycle [ISO]
- regulation of insulin secretion [IMP]
- regulation of transcription, DNA-templated [IDA, ISA]
- regulation of type B pancreatic cell development [IMP]
- response to redox state [ISO]
- spermatogenesis [IMP]
- transcription from RNA polymerase II promoter [IC]
Gene Ontology Molecular Function- DNA binding [ISO]
- E-box binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin DNA binding [IDA]
- core promoter binding [IDA]
- core promoter sequence-specific DNA binding [IDA]
- histone acetyltransferase activity [IMP, ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISA]
- transcription factor binding [ISA]
- DNA binding [ISO]
- E-box binding [IDA, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin DNA binding [IDA]
- core promoter binding [IDA]
- core promoter sequence-specific DNA binding [IDA]
- histone acetyltransferase activity [IMP, ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISA]
- transcription factor binding [ISA]
Gene Ontology Cellular Component
PER1
Gene Ontology Biological Process
- circadian regulation of gene expression [IDA, IMP, ISO]
- circadian regulation of translation [IMP]
- circadian rhythm [ISO, TAS]
- entrainment of circadian clock by photoperiod [IMP]
- histone H3 acetylation [IDA, ISO]
- histone H3 deacetylation [IMP]
- histone H4 acetylation [ISO]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- negative regulation of JNK cascade [ISO]
- negative regulation of glucocorticoid receptor signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- posttranscriptional regulation of gene expression [IMP]
- regulation of circadian rhythm [IMP]
- regulation of cytokine production involved in inflammatory response [ISO]
- regulation of hair cycle [ISO]
- regulation of p38MAPK cascade [ISO]
- regulation of sodium ion transport [IMP]
- response to cAMP [IDA]
Gene Ontology Molecular Function- E-box binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- chromatin DNA binding [IDA]
- kinase binding [IPI, ISO]
- protein binding [IPI]
- transcription factor binding [IPI]
- transcription factor binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [IDA]
- ubiquitin protein ligase binding [ISO]
- E-box binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- chromatin DNA binding [IDA]
- kinase binding [IPI, ISO]
- protein binding [IPI]
- transcription factor binding [IPI]
- transcription factor binding transcription factor activity [IDA]
- transcription regulatory region sequence-specific DNA binding [IDA]
- ubiquitin protein ligase binding [ISO]
Phenotypic Suppression
A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.
Publication
Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-BMAL1-induced transcription.
We report the cloning and mapping of mouse (mTim) and human (hTIM) orthologs of the Drosophila timeless (dtim) gene. The mammalian Tim genes are widely expressed in a variety of tissues; however, unlike Drosophila, mTim mRNA levels do not oscillate in the suprachiasmatic nucleus (SCN) or retina. Importantly, hTIM interacts with the Drosophila PERIOD (dPER) protein as well as the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PER1 CLOCK | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 676357 | |
| PER1 CLOCK | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 676342 | |
| CLOCK PER1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CLOCK PER1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PER1 CLOCK | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CLOCK PER1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.85 | BioGRID | 2683616 |
Curated By
- BioGRID