BAIT
IFNE
IFN-E, IFNE1, IFNT1, INFE1, PRO655, UNQ360/PRO655
interferon, epsilon
GO Process (13)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- B cell differentiation [IBA]
- B cell proliferation [IBA]
- T cell activation involved in immune response [IBA]
- adaptive immune response [IBA]
- cytokine-mediated signaling pathway [IBA]
- defense response to bacterium [ISS]
- defense response to virus [ISS]
- humoral immune response [IBA]
- innate immune response [IBA]
- natural killer cell activation involved in immune response [IBA]
- positive regulation of peptidyl-serine phosphorylation of STAT protein [IBA]
- regulation of MHC class I biosynthetic process [IBA]
- response to exogenous dsRNA [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CACNA2D2
CACNA2D
calcium channel, voltage-dependent, alpha 2/delta subunit 2
GO Process (3)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.944694761 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.944694761, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID