FN1
Gene Ontology Biological Process
- blood coagulation [TAS]
- cell adhesion [NAS]
- endodermal cell differentiation [IDA]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- integrin activation [IMP]
- leukocyte migration [TAS]
- peptide cross-linking [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- response to wounding [NAS]
- substrate adhesion-dependent cell spreading [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PLG
Gene Ontology Biological Process
- blood coagulation [IMP, TAS]
- cellular protein metabolic process [TAS]
- extracellular matrix disassembly [IDA, TAS]
- extracellular matrix organization [TAS]
- fibrinolysis [TAS]
- negative regulation of cell proliferation [TAS]
- negative regulation of cell-cell adhesion mediated by cadherin [TAS]
- negative regulation of cell-substrate adhesion [IDA]
- negative regulation of fibrinolysis [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of fibrinolysis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
The extracellular matrix proteins laminin and fibronectin contain binding domains for human plasminogen and tissue plasminogen activator.
This study describes the binding of plasminogen and tissue-type plasminogen activator (t-PA) to the extracellular matrix proteins fibronectin and laminin. Plasminogen bound specifically and saturably to both fibronectin and laminin immobilized on microtiter wells, with Kd(app) values of 115 and 18 nM, respectively. Limited proteolysis by endoproteinase V8 coupled with ligand blotting analysis showed that both plasminogen and t-PA preferentially ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID