DNA2
Gene Ontology Biological Process
- ATP catabolic process [IDA, TAS]
- DNA catabolic process, endonucleolytic [IDA]
- DNA double-strand break processing [IDA]
- DNA duplex unwinding [IDA]
- DNA replication [IMP]
- DNA replication checkpoint [IMP]
- DNA replication, Okazaki fragment processing [IDA]
- DNA replication, removal of RNA primer [IDA]
- DNA strand elongation involved in DNA replication [TAS]
- base-excision repair [IDA]
- mitochondrial DNA repair [IDA]
- mitochondrial DNA replication [IDA]
- mitotic cell cycle [TAS]
- positive regulation of DNA replication [IDA]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
Gene Ontology Molecular Function- 5'-3' DNA helicase activity [IDA]
- 5'-flap endonuclease activity [IDA]
- ATPase activity [TAS]
- DNA binding [IDA]
- DNA helicase activity [IDA]
- helicase activity [TAS]
- nuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA-dependent ATPase activity [IDA]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
- 5'-3' DNA helicase activity [IDA]
- 5'-flap endonuclease activity [IDA]
- ATPase activity [TAS]
- DNA binding [IDA]
- DNA helicase activity [IDA]
- helicase activity [TAS]
- nuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA-dependent ATPase activity [IDA]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
Gene Ontology Cellular Component
NRBP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.774080785 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.774080785, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DNA2 NRBP1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.8615 | BioGRID | 2262989 | |
| NRBP1 DNA2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9133 | BioGRID | 3132367 |
Curated By
- BioGRID