C17ORF62
Gene Ontology Molecular Function
P2RX4
Gene Ontology Biological Process
- apoptotic signaling pathway [IDA]
- cation transmembrane transport [IDA]
- cellular response to ATP [IDA]
- endothelial cell activation [TAS]
- ion transmembrane transport [IDA]
- membrane depolarization [IDA]
- negative regulation of cardiac muscle hypertrophy [IMP]
- positive regulation of calcium ion transport [NAS]
- positive regulation of calcium ion transport into cytosol [IDA, IMP]
- positive regulation of calcium-mediated signaling [IDA, IMP]
- positive regulation of nitric oxide biosynthetic process [NAS]
- positive regulation of prostaglandin secretion [NAS]
- purinergic nucleotide receptor signaling pathway [IMP]
- regulation of blood pressure [IMP]
- regulation of cardiac muscle contraction [IMP]
- regulation of sodium ion transport [ISS]
- relaxation of cardiac muscle [IMP]
- response to ATP [IDA]
- response to fluid shear stress [IDA]
- sensory perception of pain [ISS]
- signal transduction [IDA]
- tissue homeostasis [NAS]
- transport [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.881644575 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.881644575, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| P2RX4 C17ORF62 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9999 | BioGRID | 1190631 | |
| P2RX4 C17ORF62 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2224077 | |
| P2RX4 C17ORF62 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3095182 |
Curated By
- BioGRID