RUNX1
Gene Ontology Biological Process
- hematopoietic stem cell proliferation [TAS]
- hemopoiesis [IDA, TAS]
- myeloid cell differentiation [IDA]
- negative regulation of granulocyte differentiation [IMP]
- peripheral nervous system neuron development [TAS]
- positive regulation of angiogenesis [ISS]
- positive regulation of granulocyte differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA, IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- calcium ion binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- regulatory region DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IDA]
- DNA binding [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA, IMP]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- calcium ion binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA]
- regulatory region DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IDA]
Gene Ontology Cellular Component
CBFA2T2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The AML1-MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR) family, MTGR1.
The AML1-CBFbeta transcription factor complex is essential for the definitive hematopoiesis of all lineages and is the most frequent target of chromosomal rearrangements in human leukemia. In the t(8;21) translocation associated with acute myeloid leukemia (AML), the AML1(CBFA2/PEBP2alphaB) gene is juxtaposed to the MTG8(ETO/CDR) gene. We show here that the resultant AML1-MTG8 gene product specifically and strongly interacts with an ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RUNX1 CBFA2T2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 668597 |
Curated By
- BioGRID