BAIT
ITGA5
CD49e, FNRA, VLA5A
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
GO Process (13)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
- angiogenesis [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell-substrate adhesion [IMP]
- endodermal cell differentiation [IMP]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- leukocyte migration [TAS]
- negative regulation of anoikis [IMP]
- positive regulation of peptidyl-tyrosine phosphorylation [IMP]
- positive regulation of vascular endothelial growth factor receptor signaling pathway [TAS]
- wound healing, spreading of epidermal cells [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB35
H-ray, RAB1C, RAY
RAB35, member RAS oncogene family
GO Process (13)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [IBA]
- GTP catabolic process [IDA]
- Rab protein signal transduction [IBA]
- antigen processing and presentation [IMP]
- cellular response to nerve growth factor stimulus [ISS]
- cytokinesis [IMP]
- endocytic recycling [IBA]
- endosomal transport [IMP]
- intracellular protein transport [IBA]
- neuron projection development [ISS]
- plasma membrane to endosome transport [IMP]
- protein localization [IMP]
- protein localization to endosome [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.772674692 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.772674692, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID