Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Cyclin D3 activates Caspase 2, connecting cell proliferation with cell death.

Mendelsohn AR, Hamer JD, Wang ZB, Brent R

Precancerous cells that enter S phase without appropriate growth and viability factors undergo programmed cell death, suggesting that apoptosis may help guarantee organismic integrity [Evan, G. & Littlewood, T. (1998) Science 281, 1317-1322]. However, the connection between proliferation and cell death has remained unclear. Here, we show that the positive cell cycle regulator cyclin D3 [Matsushime H., Roussel M. F., ... [more]

Proc. Natl. Acad. Sci. U.S.A. May. 14, 2002; 99(10);6871-6 [Pubmed: 12011445]

Throughput

  • Low Throughput

Curated By

  • BioGRID