DLGAP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SHANK1
Gene Ontology Biological Process
- adult behavior [ISO]
- associative learning [ISO, ISS]
- cytoskeletal anchoring at plasma membrane [NAS]
- dendritic spine morphogenesis [ISO, ISS]
- determination of affect [ISO]
- habituation [ISO, ISS]
- long-term memory [ISO, ISS]
- negative regulation of actin filament bundle assembly [ISO, ISS]
- neuromuscular process controlling balance [ISO, ISS]
- olfactory behavior [ISO, ISS]
- positive regulation of dendritic spine development [IDA]
- positive regulation of excitatory postsynaptic membrane potential [ISO, ISS]
- protein complex assembly [IDA]
- protein localization to synapse [ISO, ISS]
- regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [ISO, ISS]
- righting reflex [ISO, ISS]
- social behavior [ISO]
- synapse maturation [IDA]
- vocalization behavior [ISO]
Gene Ontology Molecular Function- GKAP/Homer scaffold activity [IDA, ISO]
- SH3 domain binding [IPI]
- ankyrin repeat binding [IPI]
- identical protein binding [IPI]
- ionotropic glutamate receptor binding [IPI, ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- receptor signaling complex scaffold activity [IDA]
- scaffold protein binding [IPI]
- somatostatin receptor binding [IPI]
- GKAP/Homer scaffold activity [IDA, ISO]
- SH3 domain binding [IPI]
- ankyrin repeat binding [IPI]
- identical protein binding [IPI]
- ionotropic glutamate receptor binding [IPI, ISO]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein complex binding [IPI]
- receptor signaling complex scaffold activity [IDA]
- scaffold protein binding [IPI]
- somatostatin receptor binding [IPI]
Gene Ontology Cellular Component
- N-methyl-D-aspartate selective glutamate receptor complex [IDA]
- dendrite [ISO, NAS]
- dendritic spine [IDA, ISO]
- excitatory synapse [ISO, ISS]
- intracellular [NAS]
- ionotropic glutamate receptor complex [ISO, ISS]
- membrane [ISO]
- neuron projection [ISO, ISS]
- neuronal postsynaptic density [ISO, ISS]
- plasma membrane [ISO, ISS]
- postsynaptic membrane [IDA, ISO]
- synapse [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin.
NMDA receptors are linked to intracellular cytoskeletal and signaling molecules via the PSD-95 protein complex. We report a novel family of postsynaptic density (PSD) proteins, termed Shank, that binds via its PDZ domain to the C terminus of PSD-95-associated protein GKAP. A ternary complex of Shank/GKAP/PSD-95 assembles in heterologous cells and can be coimmunoprecipitated from rat brain. Synaptic localization of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SHANK1 DLGAP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| DLGAP1 SHANK1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID