BAIT
ADAMTS12
PRO4389, UNQ1918
ADAM metallopeptidase with thrombospondin type 1 motif, 12
GO Process (13)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- cell migration [IMP]
- cell-matrix adhesion [IMP]
- cellular response to BMP stimulus [IDA]
- cellular response to interleukin-1 [IMP]
- cellular response to tumor necrosis factor [IMP]
- negative regulation of cellular response to hepatocyte growth factor stimulus [IDA]
- negative regulation of cellular response to vascular endothelial growth factor stimulus [IDA]
- negative regulation of chondrocyte differentiation [IDA]
- negative regulation of hepatocyte growth factor receptor signaling pathway [IDA]
- proteoglycan catabolic process [IDA]
- proteolysis involved in cellular protein catabolic process [IDA, IMP]
- regulation of endothelial tube morphogenesis [IDA]
- regulation of inflammatory response [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
OLFM2
NOE2, NOELIN2, NOELIN2_V1, OlfC
olfactomedin 2
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.98008833 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.98008833, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID