An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phospholipase C-delta1 and oxytocin receptor signalling: evidence of its role as an effector.

Park ES, Won JH, Han KJ, Suh PG, Ryu SH, Lee HS, Yun HY, Kwon NS, Baek KJ

Although the oxytocin receptor modulates intracellular Ca2+ ion levels in myometrium, the identities of signal molecules have not been clearly clarified. Our previous studies on oxytocin receptor signalling demonstrated that 80 kDa Ghalpha is a signal mediator [Baek, Kwon, Lee, Kim, Muralidhar and Im (1996) Biochem. J. 315, 739-744]. To elucidate the effector in the oxytocin receptor signalling pathway, we ... [more]

Biochem. J. Apr. 01, 1998; 331(0);283-9 [Pubmed: 9512491]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TGM2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein-glutamine gamma-glutamyltransferase activity [IMP, ISS]

Gene Ontology Cellular Component

PLCD1