BAIT
CSRP3
CLP, CMD1M, CMH12, CRP3, LMO4, MLP
cysteine and glycine-rich protein 3 (cardiac LIM protein)
GO Process (9)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
- cardiac muscle contraction [IMP]
- cardiac muscle hypertrophy [IMP]
- cardiac muscle tissue development [ISS]
- cardiac myofibril assembly [ISS]
- cellular calcium ion homeostasis [ISS]
- detection of muscle stretch [IMP]
- protein localization to organelle [IMP]
- regulation of the force of heart contraction [ISS]
- skeletal muscle tissue development [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
GNB2
guanine nucleotide binding protein (G protein), beta polypeptide 2
GO Process (6)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.823485695 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.823485695, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID