BAIT
CITED1
MSG1
Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 1
GO Process (33)
GO Function (9)
GO Component (3)
Gene Ontology Biological Process
- SMAD protein signal transduction [IDA]
- branching involved in ureteric bud morphogenesis [ISS]
- cell proliferation [TAS]
- melanin biosynthetic process [ISS]
- melanocyte differentiation [IMP]
- mesenchymal to epithelial transition [TAS]
- metanephros development [TAS]
- negative regulation of Wnt signaling pathway [TAS]
- negative regulation of neuron apoptotic process [IDA]
- negative regulation of osteoblast differentiation [ISS]
- negative regulation of transcription, DNA-templated [IDA, ISS]
- nucleocytoplasmic transport [IDA]
- pigmentation [IMP]
- placenta development [ISS]
- positive regulation of gene expression [ISS]
- positive regulation of transcription, DNA-templated [IDA]
- positive regulation of transforming growth factor beta receptor signaling pathway [TAS]
- regulation of transcription from RNA polymerase II promoter [TAS]
- response to cAMP [ISS]
- response to cytokine [ISS]
- response to estrogen [IDA, TAS]
- response to insulin [ISS]
- response to interferon-gamma [ISS]
- response to interleukin-1 [ISS]
- response to interleukin-11 [ISS]
- response to interleukin-2 [ISS]
- response to interleukin-4 [ISS]
- response to interleukin-6 [ISS]
- response to interleukin-9 [ISS]
- response to lipopolysaccharide [ISS]
- response to parathyroid hormone [ISS]
- response to transforming growth factor beta [IDA]
- transforming growth factor beta receptor signaling pathway [IDA]
Gene Ontology Molecular Function- LBD domain binding [IDA]
- chromatin binding [ISS]
- co-SMAD binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
- LBD domain binding [IDA]
- chromatin binding [ISS]
- co-SMAD binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
Homo sapiens
PREY
EGLN1
C1orf12, ECYT3, HIF-PH2, HIFPH2, HPH-2, HPH2, PHD2, SM20, ZMYND6, PNAS-118
egl-9 family hypoxia-inducible factor 1
GO Process (10)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- cellular response to hypoxia [TAS]
- negative regulation of cAMP catabolic process [ISS]
- negative regulation of cyclic-nucleotide phosphodiesterase activity [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- oxygen homeostasis [IDA]
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline [IDA]
- regulation of angiogenesis [ISS]
- regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]
- response to hypoxia [IDA]
- response to nitric oxide [IDA]
Gene Ontology Molecular Function- enzyme binding [ISS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- peptidyl-proline dioxygenase activity [TAS]
- protein binding [IPI]
- enzyme binding [ISS]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors [IDA]
- peptidyl-proline 4-dioxygenase activity [IDA]
- peptidyl-proline dioxygenase activity [TAS]
- protein binding [IPI]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999649648 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.999649648, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID