BAIT
SSBP2
HSPC116, SOSS-B2
single-stranded DNA binding protein 2
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
ALOX15
12-LOX, 15-LOX-1, 15LOX-1
arachidonate 15-lipoxygenase
GO Process (21)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- apoptotic cell clearance [ISS]
- arachidonic acid metabolic process [IDA, NAS, TAS]
- bone mineralization [ISS]
- cellular response to calcium ion [IDA]
- cellular response to interleukin-13 [IMP]
- hepoxilin biosynthetic process [ISS]
- inflammatory response [TAS]
- leukotriene metabolic process [TAS]
- lipoxin A4 biosynthetic process [ISS]
- lipoxygenase pathway [IDA, TAS]
- negative regulation of adaptive immune response [ISS]
- ossification [ISS]
- phosphatidylethanolamine biosynthetic process [ISS]
- positive regulation of ERK1 and ERK2 cascade [IMP]
- positive regulation of actin filament polymerization [ISS]
- positive regulation of cell-substrate adhesion [IDA]
- regulation of engulfment of apoptotic cell [ISS]
- regulation of peroxisome proliferator activated receptor signaling pathway [ISS]
- response to endoplasmic reticulum stress [ISS]
- small molecule metabolic process [TAS]
- wound healing [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999999981 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.999999981, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID