BAIT
TRDN
CPVT5, TDN, TRISK, RP11-167I10.1
triadin
GO Process (15)
GO Function (4)
GO Component (11)
Gene Ontology Biological Process
- cellular calcium ion homeostasis [ISS]
- cytoplasmic microtubule organization [ISS]
- endoplasmic reticulum membrane organization [ISS]
- heart contraction [IMP]
- ion transmembrane transport [TAS]
- muscle contraction [TAS]
- negative regulation of ryanodine-sensitive calcium-release channel activity [TAS]
- positive regulation of cell communication by electrical coupling involved in cardiac conduction [ISS]
- positive regulation of ryanodine-sensitive calcium-release channel activity [ISS, TAS]
- regulation of cardiac muscle cell membrane potential [ISS]
- regulation of cell communication by electrical coupling [TAS]
- regulation of release of sequestered calcium ion into cytosol [TAS]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISS]
- release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [ISS]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- calcium channel complex [ISS]
- cytoplasm [IDA]
- endoplasmic reticulum [ISS]
- integral component of membrane [TAS]
- junctional membrane complex [ISS]
- junctional sarcoplasmic reticulum membrane [ISS, TAS]
- plasma membrane [IDA]
- sarcoplasmic reticulum [ISS]
- sarcoplasmic reticulum lumen [TAS]
- sarcoplasmic reticulum membrane [TAS]
- voltage-gated calcium channel complex [ISS]
Homo sapiens
PREY
RHOT1
ARHT1, MIRO-1, MIRO1
ras homolog family member T1
GO Process (5)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.762782289 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.762782289, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID