BAIT
WNT3A
wingless-type MMTV integration site family, member 3A
GO Process (22)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- COP9 signalosome assembly [ISS]
- canonical Wnt signaling pathway [IDA]
- canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment [IDA]
- cell proliferation in forebrain [ISS]
- cellular response to retinoic acid [ISS]
- negative regulation of heart induction by canonical Wnt signaling pathway [IDA]
- negative regulation of neurogenesis [ISS]
- negative regulation of neuron projection development [ISS]
- neuron differentiation [IBA, ISS]
- palate development [IMP]
- positive regulation of canonical Wnt signaling pathway [IDA]
- positive regulation of cardiac muscle cell differentiation [IDA]
- positive regulation of catenin import into nucleus [IDA]
- positive regulation of cell proliferation [ISS]
- positive regulation of dermatome development [IDA]
- positive regulation of mesodermal cell fate specification [IDA]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of protein binding [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of receptor internalization [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
LDLR
FH, FHC, LDLCQ2
low density lipoprotein receptor
GO Process (15)
GO Function (5)
GO Component (13)
Gene Ontology Biological Process
- cholesterol homeostasis [IMP]
- cholesterol import [ISS]
- cholesterol transport [IMP]
- endocytosis [TAS]
- intestinal cholesterol absorption [IMP]
- lipid metabolic process [TAS]
- lipoprotein metabolic process [TAS]
- low-density lipoprotein particle clearance [IMP]
- phospholipid transport [ISS]
- phototransduction, visible light [TAS]
- positive regulation of triglyceride biosynthetic process [ISS]
- receptor-mediated endocytosis [TAS]
- regulation of phosphatidylcholine catabolic process [ISS]
- retinoid metabolic process [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cell surface [IDA]
- clathrin-coated endocytic vesicle membrane [TAS]
- coated pit [IDA]
- early endosome [IDA]
- endosome membrane [TAS]
- external side of plasma membrane [IDA]
- integral component of plasma membrane [TAS]
- late endosome [IDA]
- lysosome [IDA]
- membrane [IDA]
- plasma membrane [TAS]
- receptor complex [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.964293662 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.964293662, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID