BAIT
POLR1D
AC19, POLR1C, RPA16, RPA9, RPAC2, RPC16, RPO1-3, TCS2
polymerase (RNA) I polypeptide D, 16kDa
GO Process (10)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
- gene expression [TAS]
- innate immune response [TAS]
- positive regulation of type I interferon production [TAS]
- termination of RNA polymerase I transcription [TAS]
- termination of RNA polymerase III transcription [TAS]
- transcription elongation from RNA polymerase I promoter [TAS]
- transcription elongation from RNA polymerase III promoter [TAS]
- transcription from RNA polymerase I promoter [TAS]
- transcription from RNA polymerase III promoter [TAS]
- transcription initiation from RNA polymerase I promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TUBA1A
B-ALPHA-1, LIS3, TUBA3
tubulin, alpha 1a
GO Process (8)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.857097591 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.857097591, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID