BAIT
LGR4
BNMD17, GPR48
leucine-rich repeat containing G protein-coupled receptor 4
GO Process (8)
GO Function (3)
GO Component (1)
Gene Ontology Biological Process
- bone mineralization [ISS]
- bone remodeling [ISS]
- circadian regulation of gene expression [ISS]
- negative regulation of cytokine secretion [ISS]
- negative regulation of toll-like receptor signaling pathway [ISS]
- osteoblast differentiation [ISS]
- positive regulation of canonical Wnt signaling pathway [IDA]
- spermatogenesis [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SLC27A1
ACSVL5, FATP, FATP1
solute carrier family 27 (fatty acid transporter), member 1
GO Process (12)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
- cardiolipin biosynthetic process [IMP]
- cellular lipid metabolic process [TAS]
- long-chain fatty acid transport [TAS]
- negative regulation of phospholipid biosynthetic process [IMP]
- phosphatidic acid biosynthetic process [IMP]
- phosphatidylcholine biosynthetic process [IMP]
- phosphatidylethanolamine biosynthetic process [IMP]
- phosphatidylglycerol biosynthetic process [IMP]
- phosphatidylinositol biosynthetic process [IMP]
- phosphatidylserine biosynthetic process [IMP]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.932767185 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.932767185, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID