BAIT
FFAR1
FFA1R, GPCR40, GPR40
free fatty acid receptor 1
GO Process (6)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NDUFA13
B16.6, CDA016, GRIM-19, GRIM19, CGI-39
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
GO Process (14)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
- apoptotic signaling pathway [IDA]
- cellular metabolic process [TAS]
- cellular response to interferon-beta [IDA]
- cellular response to retinoic acid [IDA]
- negative regulation of cell growth [IDA, IMP]
- negative regulation of intrinsic apoptotic signaling pathway [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IGI]
- positive regulation of peptidase activity [IC]
- positive regulation of protein catabolic process [IGI]
- protein import into mitochondrial inner membrane [IDA]
- reactive oxygen species metabolic process [IMP]
- respiratory electron transport chain [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.78511442 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.78511442, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID