BAIT
NPPB
BNP
natriuretic peptide B
GO Process (11)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- body fluid secretion [TAS]
- cGMP biosynthetic process [IDA]
- cell surface receptor signaling pathway [NAS]
- negative regulation of angiogenesis [TAS]
- negative regulation of cell growth [NAS]
- positive regulation of renal sodium excretion [TAS]
- positive regulation of urine volume [TAS]
- receptor guanylyl cyclase signaling pathway [IDA]
- regulation of blood pressure [NAS]
- regulation of vascular permeability [TAS]
- regulation of vasodilation [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CASK
CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8, RP11-540L11.1
calcium/calmodulin-dependent serine protein kinase (MAGUK family)
GO Process (7)
GO Function (2)
GO Component (11)
Gene Ontology Biological Process
- cell adhesion [TAS]
- extracellular matrix organization [TAS]
- negative regulation of cell-matrix adhesion [IMP]
- negative regulation of cellular response to growth factor stimulus [IMP]
- negative regulation of keratinocyte proliferation [IMP]
- negative regulation of wound healing [IMP]
- nucleotide phosphorylation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.931211304 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.931211304, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID