BAIT
HLA-DRB1
DRB1, DRw10, HLA-DR1B, HLA-DRB, SS1, XXbac-BPG161M6.1
major histocompatibility complex, class II, DR beta 1
GO Process (19)
GO Function (3)
GO Component (16)
Gene Ontology Biological Process
- T cell costimulation [TAS]
- T cell receptor signaling pathway [TAS]
- T-helper 1 type immune response [ISS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- cytokine-mediated signaling pathway [TAS]
- detection of bacterium [ISS]
- humoral immune response mediated by circulating immunoglobulin [IDA, ISS]
- immune response [ISS, NAS]
- immunoglobulin production involved in immunoglobulin mediated immune response [IDA, ISS]
- inflammatory response to antigenic stimulus [ISS]
- interferon-gamma-mediated signaling pathway [TAS]
- negative regulation of T cell proliferation [ISS]
- negative regulation of interferon-gamma production [ISS]
- peptide antigen assembly with MHC class II protein complex [IDA]
- polysaccharide assembly with MHC class II protein complex [IDA]
- positive regulation of insulin secretion involved in cellular response to glucose stimulus [ISS]
- protein tetramerization [ISS]
- regulation of interleukin-10 secretion [ISS]
- regulation of interleukin-4 production [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER to Golgi transport vesicle membrane [TAS]
- Golgi membrane [TAS]
- MHC class II protein complex [IDA, ISS]
- cell surface [IDA]
- clathrin-coated endocytic vesicle membrane [TAS]
- endocytic vesicle membrane [TAS]
- external side of plasma membrane [ISS]
- extracellular vesicular exosome [IDA]
- integral component of lumenal side of endoplasmic reticulum membrane [TAS]
- integral component of plasma membrane [TAS]
- late endosome membrane [IDA]
- lysosomal membrane [IDA, TAS]
- membrane [IDA, NAS]
- plasma membrane [TAS]
- trans-Golgi network membrane [TAS]
- transport vesicle membrane [TAS]
Homo sapiens
PREY
ATP12A
ATP1AL1
ATPase, H+/K+ transporting, nongastric, alpha polypeptide
GO Process (2)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.999837617 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.999837617, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID