BCL2L12
Gene Ontology Biological Process
Gene Ontology Molecular Function
BAX
Gene Ontology Biological Process
- B cell apoptotic process [IDA]
- B cell receptor apoptotic signaling pathway [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c [IDA]
- apoptotic mitochondrial changes [IDA]
- apoptotic process [NAS, TAS]
- apoptotic signaling pathway [IDA]
- endoplasmic reticulum calcium ion homeostasis [TAS]
- establishment or maintenance of transmembrane electrochemical gradient [IDA]
- extrinsic apoptotic signaling pathway [IDA]
- extrinsic apoptotic signaling pathway in absence of ligand [IBA]
- extrinsic apoptotic signaling pathway via death domain receptors [IC]
- intrinsic apoptotic signaling pathway [IDA, TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IBA]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IMP]
- mitochondrial fragmentation involved in apoptotic process [IDA]
- mitochondrial fusion [IDA]
- negative regulation of protein binding [IDA]
- positive regulation of apoptotic DNA fragmentation [IMP]
- positive regulation of apoptotic process [IMP]
- positive regulation of endoplasmic reticulum unfolded protein response [IMP]
- positive regulation of intrinsic apoptotic signaling pathway [IMP]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [TAS]
- positive regulation of neuron apoptotic process [IDA]
- positive regulation of protein oligomerization [IDA]
- positive regulation of release of cytochrome c from mitochondria [IDA]
- protein homooligomerization [IDA]
- protein oligomerization [IDA]
- regulation of mitochondrial membrane potential [IDA]
- regulation of protein heterodimerization activity [IPI]
- regulation of protein homodimerization activity [IDA]
- release of cytochrome c from mitochondria [IDA]
- release of matrix enzymes from mitochondria [IDA]
- response to toxic substance [IDA]
- retinal cell apoptotic process [IMP]
- transformed cell apoptotic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BAX complex [IDA]
- Bcl-2 family protein complex [IDA]
- cytosol [IDA, TAS]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum membrane [IDA]
- extracellular vesicular exosome [IDA]
- membrane [IDA]
- mitochondrial outer membrane [IBA, TAS]
- mitochondrial permeability transition pore complex [IDA]
- mitochondrion [IDA]
- nuclear envelope [IDA]
- nucleus [IDA, IMP]
- pore complex [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Quantitative Score
- 0.982179305 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.982179305, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BCL2L12 BAX | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9985 | BioGRID | 1176691 | |
| BCL2L12 BAX | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9959 | BioGRID | 2237145 | |
| BAX BCL2L12 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | 0.0382 | BioGRID | 3584489 | |
| BCL2L12 BAX | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| BAX BCL2L12 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID