BAIT
CCDC22
CXorf37, JM1
coiled-coil domain containing 22
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
TRIM16
EBBP
tripartite motif containing 16
GO Process (9)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- histone H3 acetylation [IDA]
- histone H4 acetylation [IDA]
- positive regulation of interleukin-1 beta secretion [IMP]
- positive regulation of keratinocyte differentiation [IDA]
- positive regulation of retinoic acid receptor signaling pathway [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- response to growth hormone [IDA]
- response to organophosphorus [IEP]
- response to retinoic acid [IEP]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-specific interaction networks. The first, BioPlex 3.0, results from affinity purification of 10,128 human proteins-half the proteome-in 293T cells and includes 118,162 interactions among 14,586 proteins. The second results from 5,522 immunoprecipitations in HCT116 ... [more]
Cell May. 27, 2021; 184(11);3022-3040.e28 [Pubmed: 33961781]
Quantitative Score
- 0.986209269 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex HCT HCT116 cells CompPASS score = 0.986209269, threshold = 0.75. Quantitative scores are calculated by CompPASS-Plus (Huttlin et al. Cell 2015, PMID: 26186194). The 0.75 threshold represents the top 2% of scores in HCT116.
- Only scores from within the same cell line in BioPlex HCT (PMID: 33961781) should be compared directly. For comparison of HEK293T and HCT116 interaction networks with relaxed threshold = 0.1, see BioPlex Interactome (https://bioplex.hms.harvard.edu/index.php).
Curated By
- BioGRID