DCC1
Gene Ontology Biological Process
Gene Ontology Cellular Component
POL2
Gene Ontology Biological Process
- DNA replication proofreading [IMP]
- DNA-dependent DNA replication [IDA]
- base-excision repair [IMP]
- double-strand break repair [IMP]
- double-strand break repair via nonhomologous end joining [IGI, IMP]
- error-prone translesion synthesis [IDA]
- gene conversion [IMP]
- heterochromatin organization involved in chromatin silencing [IGI, IMP]
- intra-S DNA damage checkpoint [IGI, IMP, IPI]
- leading strand elongation [IMP]
- mitotic DNA replication checkpoint [IMP]
- mitotic sister chromatid cohesion [IMP]
- nucleotide-excision repair, DNA gap filling [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Ctf18-RFC and DNA Pol ? form a stable leading strand polymerase/clamp loader complex required for normal and perturbed DNA replication.
The eukaryotic replisome must faithfully replicate DNA and cope with replication fork blocks and stalling, while simultaneously promoting sister chromatid cohesion. Ctf18-RFC is an alternative PCNA loader that links all these processes together by an unknown mechanism. Here, we use integrative structural biology combined with yeast genetics and biochemistry to highlight the specific functions that Ctf18-RFC plays within the leading ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DCC1 POL2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| POL2 DCC1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.2523 | BioGRID | 2012177 | |
| POL2 DCC1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 1518610 |
Curated By
- BioGRID