CCAR2
Gene Ontology Biological Process
- RNA splicing [ISO]
- mitochondrial fragmentation involved in apoptotic process [ISO]
- negative regulation of catalytic activity [IDA, ISO]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [ISO]
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of DNA damage checkpoint [ISO]
- positive regulation of apoptotic process [ISO]
- regulation of DNA-templated transcription, elongation [ISO]
- regulation of circadian rhythm [IMP]
- regulation of protein stability [ISO]
- response to UV [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GAPDH
Gene Ontology Biological Process
- apoptotic process [ISO]
- carbohydrate metabolic process [ISO]
- cellular response to interferon-gamma [ISO]
- gluconeogenesis [ISO]
- glycolytic process [ISO]
- microtubule cytoskeleton organization [ISO]
- multicellular organismal development [IMP]
- negative regulation of translation [ISO]
- neuron apoptotic process [ISO]
- oxidation-reduction process [IMP]
- peptidyl-cysteine S-trans-nitrosylation [ISO]
- protein stabilization [ISO]
- response to ammonium ion [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [IDA, ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- membrane [ISO]
- microtubule cytoskeleton [ISO]
- mitochondrion [IDA]
- nuclear membrane [ISO]
- nucleus [ISO]
- plasma membrane [ISO]
- ribonucleoprotein complex [ISO]
- vesicle [ISO]
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Hydrogen sulfide-induced GAPDH sulfhydration disrupts the CCAR2-SIRT1 interaction to initiate autophagy.
The deacetylase SIRT1 (sirtuin 1) has emerged as a major regulator of nucleocytoplasmic distribution of macroautophagy/autophagy marker MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3). Activation of SIRT1 leads to the deacetylation of LC3 and its translocation from the nucleus into the cytoplasm leading to an increase in the autophagy flux. Notably, hydrogen sulfide (H2S) is a cytoprotective gasotransmitter known to ... [more]
Throughput
- Low Throughput
Additional Notes
- Proximity Ligation Assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GAPDH CCAR2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID