An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

USP11 mediates repair of DNA-protein cross-links by deubiquitinating SPRTN metalloprotease.

Perry M, Biegert M, Kollala SS, Mallard H, Su G, Kodavati M, Kreiling N, Holbrook A, Ghosal G

DNA-protein cross-links (DPCs) are toxic DNA lesions that interfere with DNA metabolic processes such as replication, transcription, and recombination. USP11 deubiquitinase participates in DNA repair, but the role of USP11 in DPC repair is not known. SPRTN is a replication-coupled DNA-dependent metalloprotease that cleaves proteins cross-linked to DNA to promote DPC repair. SPRTN function is tightly regulated by a monoubiquitin ... [more]

J Biol Chem Feb. 11, 2021; 296();100396 [Pubmed: 33567341]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TUBB3 USP11	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Stockum A (2018)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

USP11

Gene Ontology Biological Process

Gene Ontology Molecular Function

cysteine-type endopeptidase activity [TAS]protein binding [IPI]ubiquitin thiolesterase activity [IBA]ubiquitin-specific protease activity [IDA]

Gene Ontology Cellular Component

TUBB3