Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Tau knockout exacerbates degeneration of parvalbumin-positive neurons in substantia nigra pars reticulata in Parkinson's disease-related ?-synuclein A53T mice.

Jiao L, Zheng M, Duan J, Wu T, Li Z, Liu L, Xiang X, Tang X, He J, Li X, Zhang G, Ding J, Cai H, Lin X

?-Synuclein (?-syn)-induced neurotoxicity has been generally accepted as a key step in the pathogenesis of Parkinson's disease (PD). Microtubule-associated protein tau, which is considered second only to ?-syn, has been repeatedly linked with PD in association studies. However, the underlying interaction between these two PD-related proteins in vivo remains unclear. To investigate how the expression of tau affects ?-syn-induced neurodegeneration ... [more]

FASEB J Dec. 01, 2019; 34(9);12239-12254 [Pubmed: 33000527]

Throughput

  • Low Throughput

Curated By

  • BioGRID