BAIT

RALGPS2

1810020P17Rik, 2210408F11Rik, 4921528G01Rik, 9130014M22Rik, AU043409, AW046161
Ral GEF with PH domain and SH3 binding motif 2
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY

RALA

3010001O15Rik, AW322615, Ral, Rasl1, RP23-5P19.1
v-ral simian leukemia viral oncogene homolog A (ras related)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Functional analysis of RalGPS2, a murine guanine nucleotide exchange factor for RalA GTPase.

Ceriani M, Scandiuzzi C, Amigoni L, Tisi R, Berruti G, Martegani E

RalGPS2 is a murine guanine nucleotide exchange factor of the RalGPS family; it contains a Cdc25-like GEF domain and does not exhibit a Ras-binding domain. The main characteristic of RalGPS2 is its pleckstrin homology (PH) domain, present at the C terminus, that preferentially binds phosphatidylinositol-4,5-biphosphate and in HEK 293 cells localized in membranes, causing ruffling and vesiculation. Moreover, RalGPS2 contains ... [more]

Exp Cell Res Jul. 01, 2007; 313(11);2293-307 [Pubmed: 17462626]

Throughput

  • Low Throughput

Curated By

  • BioGRID