RALGPS2
GRB2
Gene Ontology Biological Process
- Ras protein signal transduction [TAS]
- aging [ISO]
- anatomical structure formation involved in morphogenesis [IMP]
- branching involved in labyrinthine layer morphogenesis [IMP]
- cell differentiation [IMP]
- cellular response to ionizing radiation [ISO]
- fibroblast growth factor receptor signaling pathway [IGI]
- insulin receptor signaling pathway [ISO]
- positive regulation of actin filament polymerization [IGI]
- positive regulation of reactive oxygen species metabolic process [ISO]
- positive regulation of signal transduction [IDA]
- protein heterooligomerization [ISO]
- receptor internalization [ISO]
- regulation of MAPK cascade [IGI]
- signal transduction in response to DNA damage [ISO]
Gene Ontology Molecular Function- SH3 domain binding [ISO]
- SH3/SH2 adaptor activity [IDA]
- ephrin receptor binding [IPI, ISO]
- epidermal growth factor receptor binding [ISO]
- identical protein binding [ISO]
- insulin receptor substrate binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphoprotein binding [ISO]
- phosphotyrosine binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [IPI]
- SH3 domain binding [ISO]
- SH3/SH2 adaptor activity [IDA]
- ephrin receptor binding [IPI, ISO]
- epidermal growth factor receptor binding [ISO]
- identical protein binding [ISO]
- insulin receptor substrate binding [ISO]
- neurotrophin TRKA receptor binding [ISO]
- phosphoprotein binding [ISO]
- phosphotyrosine binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein domain specific binding [IPI, ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [IPI]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Functional analysis of RalGPS2, a murine guanine nucleotide exchange factor for RalA GTPase.
RalGPS2 is a murine guanine nucleotide exchange factor of the RalGPS family; it contains a Cdc25-like GEF domain and does not exhibit a Ras-binding domain. The main characteristic of RalGPS2 is its pleckstrin homology (PH) domain, present at the C terminus, that preferentially binds phosphatidylinositol-4,5-biphosphate and in HEK 293 cells localized in membranes, causing ruffling and vesiculation. Moreover, RalGPS2 contains ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RALGPS2 GRB2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID