An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

An N-terminal motif unique to primate tau enables differential protein-protein interactions.

Stefanoska K, Volkerling A, Bertz J, Poljak A, Ke YD, Ittner LM, Ittner A

Compared with other mammalian species, humans are particularly susceptible to tau-mediated neurodegenerative disorders. Differential interactions of the tau protein with other proteins are critical for mediating tau's physiological functions as well as tau-associated pathological processes. Primate tau harbors an 11-amino acid-long motif in its N-terminal region (residues 18-28), which is not present in non-primate species and whose function is unknown. ... [more]

J Biol Chem Dec. 09, 2017; 293(10);3710-3719 [Pubmed: 29382714]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

MAPT

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3 domain binding [IPI]apolipoprotein binding [IPI]enzyme binding [IPI]lipoprotein particle binding [IPI]microtubule binding [IDA]protein binding [IPI]structural constituent of cytoskeleton [TAS]

Gene Ontology Cellular Component

HSP90AA1