BAIT

CREB1

CREB
cAMP responsive element binding protein 1
GO Process (35)
GO Function (11)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

cAMP-responding element-binding protein and c-Ets1 interact in the regulation of ATP-dependent MUC5AC gene expression.

Song KS, Lee TJ, Kim K, Chung KC, Yoon JH

Exogenous ATP activates purinoreceptors on the cell surface that regulate diverse cellular functions, including mucous cell secretion in the respiratory epithelium. In this study, ATP increased MUC5AC mRNA in primary human nasal epithelial cells and in NCI-H292 pulmonary adenocarcinoma cells in vitro. ATP-induced MUC5AC mRNA was mediated by phospholipase Cbeta3. A dominant-negative mutation in the PDZ binding domain of PLCbeta3 ... [more]

J. Biol. Chem. Oct. 03, 2008; 283(40);26869-78 [Pubmed: 18676374]

Throughput

  • Low Throughput

Additional Notes

  • assayed using GST (glutathione S-transferase) pull-down experiments

Curated By

  • BioGRID