An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mammalian BCAS3 and C16orf70 associate with the phagophore assembly site in response to selective and non-selective autophagy.

Kojima W, Yamano K, Kosako H, Imai K, Kikuchi R, Tanaka K, Matsuda N

Macroautophagy/autophagy is an intracellular degradation process that delivers cytosolic materials and/or damaged organelles to lysosomes. De novo synthesis of the autophagosome membrane occurs within a phosphatidylinositol-3-phosphate-rich region of the endoplasmic reticulum, and subsequent expansion is critical for cargo encapsulation. This process is complex, especially in mammals, with many regulatory factors. In this study, by utilizing PRKN (parkin RBR E3 ubiquitin ... [more]

Autophagy Dec. 01, 2020; 17(8);2011-2036 [Pubmed: 33499712]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

ZFYVE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

1-phosphatidylinositol binding [IDA]phosphatidylinositol-3,4,5-trisphosphate binding [IDA]phosphatidylinositol-3,4-bisphosphate binding [IDA]protein binding [IPI]zinc ion binding [NAS]

Gene Ontology Cellular Component

UQCRC1