Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

An Interaction Network of the Human SEPT9 Established by Quantitative Mass Spectrometry.

Hecht M, Roesler R, Wiese S, Johnsson N, Gronemeyer T

Septins regulate the organization of the actin cytoskeleton, vesicle transport and fusion, chromosome alignment and segregation, and cytokinesis in mammalian cells. SEPT9 is part of the core septin hetero-octamer in human cells which is composed of SEPT2, SEPT6, SEPT7, and SEPT9. SEPT9 has been linked to a variety of intracellular functions as well as to diseases and diverse types of ... [more]

G3 (Bethesda) Dec. 05, 2018; 9(6);1869-1880 [Pubmed: 30975701]

Throughput

  • Low Throughput

Curated By

  • BioGRID