BAIT

PARK2

AR-JP, LPRS2, PDJ, PRKN, KB-152G3.1
parkin RBR E3 ubiquitin protein ligase
GO Process (58)
GO Function (22)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens
PREY

UBE2V1

CIR1, CROC-1, CROC1, UBE2V, UEV-1, UEV1, UEV1A, P/OKcl.19
ubiquitin-conjugating enzyme E2 variant 1
GO Process (23)
GO Function (4)
GO Component (8)
Homo sapiens

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Diverse effects of pathogenic mutations of Parkin that catalyze multiple monoubiquitylation in vitro.

Matsuda N, Kitami T, Suzuki T, Mizuno Y, Hattori N, Tanaka K

Mutational dysfunction of PARKIN gene, which encodes a double RING finger protein and has ubiquitin ligase E3 activity, is the major cause of autosomal recessive juvenile Parkinsonism. Although many studies explored the functions of Parkin, its biochemical character is poorly understood. To address this issue, we established an E3 assay system using maltose-binding protein-fused Parkin purified from Escherichia coli. Using ... [more]

J. Biol. Chem. Feb. 10, 2006; 281(6);3204-9 [Pubmed: 16339143]

Throughput

  • Low Throughput

Additional Notes

  • E2: Ubc7/UBCH7/UEV1-UBC13

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PARK2 UBE2V1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1067178
PARK2 UBE2V1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
PARK2 UBE2V1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
947909
PARK2 UBE2V1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
1067177

Curated By

  • BioGRID