Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of proteins that interact with catalytically active calcium-dependent protein kinases from Arabidopsis.

Uno Y, Rodriguez Milla MA, Maher E, Cushman JC

Calcium-dependent protein kinases (CDPKs) are essential sensor-transducers of calcium signaling pathways in plants. Functional characterization of CDPKs is of great interest because they play important roles during growth, development, and in response to a wide range of environmental stimuli. The Arabidopsis genome encodes 34 CDPKs, but very few substrates of these enzymes have been identified. In this study, we exploited ... [more]

Mol. Genet. Genomics Apr. 01, 2009; 281(4);375-90 [Pubmed: 19130088]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CPK4

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin-dependent protein kinase activity [ISS]kinase activity [ISS]protein binding [IPI]protein kinase activity [IDA]

Gene Ontology Cellular Component

PSB29